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2001, `RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension (2001)
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| Venue: | Science |
| Citations: | 61 - 9 self |
BibTeX
@ARTICLE{Johnston012001,`rna-catalyzed,
author = {Wendy K. Johnston and Peter J. Unrau and Michael S. Lawrence and Margaret E. Glasner and David P. Bartel},
title = {2001, `RNA-Catalyzed RNA Polymerization: Accurate and General RNA-Templated Primer Extension},
journal = {Science},
year = {2001},
volume = {292},
pages = {1319--1325}
}
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Abstract
The RNA world hypothesis regarding the early evolution of life relies on the premise that some RNA sequences can catalyze RNA replication. In support of this conjecture, we describe here an RNA molecule that catalyzes the type of polymerization needed for RNA replication. The ribozyme uses nucleoside triphosphates and the coding information of an RNA template to extend an RNA primer by the successive addition of up to 14 nucleotides—more than a complete turn of an RNA helix. Its polymerization activity is general in terms of the sequence and the length of the primer and template RNAs, provided that the 3 � terminus of the primer pairs with the template. Its polymerization is also quite accurate: when primers extended by 11 nucleotides were cloned and sequenced, 1088 of 1100 sequenced nucleotides matched the template. The RNA world hypothesis states that early life forms lacked protein enzymes and depended instead on enzymes composed of RNA (1). Much of the appeal of this hypothesis comes
Keyphrases
general rna-templated primer extension rna-catalyzed rna polymerization rna replication rna template early life form template rna rna world hypothesis state rna world hypothesis polymerization activity rna sequence protein enzyme early evolution successive addition complete turn rna helix primer pair life relies rna primer nucleoside triphosphates rna molecule
