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Open Access Evaluation of Antidiabetic and Antioxidant Effects of Seabuckthorn (Hippophae rhamnoides L.) in Streptozotocin-Nicotinamide Induced Diabetic Rats
BibTeX
@MISC{Sharma_openaccess,
author = {M. Sharma and M. W. Siddique and Akhter M. Shamim and Shukla Gyanesh and K. K. Pillai},
title = {Open Access Evaluation of Antidiabetic and Antioxidant Effects of Seabuckthorn (Hippophae rhamnoides L.) in Streptozotocin-Nicotinamide Induced Diabetic Rats},
year = {}
}
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Abstract
Abstract: Seabuckthorn (Hippophae rhamnoides L.) is a branched deciduous shrub native to Europe and Asia. The plant has been used extensively in oriental traditional system of medicine for treatment of different diseases. Seabuckthorn is found to have significant antioxidative, antimicrobial, immunomodulatory, cytoprotective, hepatoprtective and tissue regenerative properties. The present study was undertaken to evaluate the antidiabetic and antioxidant effect of seabuckthorn (Hippophae rhamnoides L.) in streptozotocin-nicotinamide induced type-2 diabetic rats. Experimental diabetes was induced by a single intraperitonial injection of streptozotocin (60 mg/kg), 15 minutes after the i.p. administration of 120mg/kg nicotinamide. Seabuckthorn was administered orally to streptozotocin (STZ) diabetic rats. Blood glucose, tissue glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) in pancreas were estimated following the established procedures. Biochemical observations were supplemented with histological examination of pancreatic tissue. The increase of blood glucose, TBARS level with reduction in GSH content were the salient features observed in STZ- diabetic rats. Sea buckthorn produced a significant (p< 0.05) reduction in blood glucose levels and TBARS levels in the STZ- diabetic rats. GSH, reduced significantly (p< 0.05) in diabetic rats, was brought back to near normal levels by co-administration of sea buckthorn. Degenerative changes of pancreatic beta cells in STZ- diabetic rats were minimized to near normal morphology by administration of sea buckthorn as evident by histopathological examination. The results of the study indicate the role of oxidative stress in the induction of diabetes and suggest a
Keyphrases
antioxidant effect streptozotocin-nicotinamide induced diabetic rat stz diabetic rat open access evaluation diabetic rat tbars level sea buckthorn blood glucose tissue glutathione tissue regenerative property streptozotocin-nicotinamide induced type-2 diabetic rat gsh content pancreatic beta cell biochemical observation pancreatic tissue single intraperitonial injection thiobarbituric acid reactive substance present study histopathological examination oxidative stress blood glucose level normal level branched deciduous shrub different disease histological examination normal morphology degenerative change experimental diabetes oriental traditional system
