Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21 (1992)
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BibTeX
@MISC{Ronald92geneticand,
author = {Pamela C. Ronald and Beng Albano and Rodante Tabien and Lleva Abenes and Kung-sheng Wu and Susan Mccouch and Steven D. Tanksley},
title = {Genetic and physical analysis of the rice bacterial blight disease resistance locus, Xa21},
year = {1992}
}
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Abstract
Summary. Nearly isogenic lines (NILs) of rice (Oryza sativa) differing at a locus conferring resistance to the pathogen Xanthomonas oryzae pv. oryzae were surveyed with 123 DNA markers and 985 random primers using restriction fragment length plymorphism (RFLP) and random amplified polymorphic DNA (RAPD) analysis. One chromosome 11 marker (RG103) detected polymor-phism between the NILs that cosegregated with Xa21. All other chromosome 11 DNA markers tested were monomorphic between the NILs, localizing the Xa21 in-trogressed region to an 8.3 cM interval on chromosome 11. Furthermore, we identified two polymerase chain re-action (PCR) products (RAPD2148 and RAPD818) that detected polymorphisms between the NILs. Genomic se-quences hybridizing with RAPD818, RAPD248 and RG103 were duplicated specifically in the Xa21 NIL. All three markers cosegregated with the resistance locus, Xa21, in a F2 population of 386 progeny. Based on the frequency with which we recovered polymorphic Xa21-linked markers, we estimated the physical size of the introgressed region to be approximately 800 kb. This estimation was supported by physical mapping (using pulsed field gel electrophoresis) of the sequences hybrid-izing with the three J(a21-1inked DNA markers. The r-sults showed that the three Xa21-1inked markers are physically close to each other, with one copy of the RAPD818 sequences located within 60 kb of RAPD248 and the other copy within 270 kb of RGI03. None of the enzymes tested generated a DNA fragment hat hy-bridized with all three of the markers indicating that the introgressed region containing the r sistance locus Xa21 is probably larger than 270 kb.
Keyphrases
physical analysis dna marker introgressed region genomic se-quences physical mapping cm interval random amplified polymorphic dna xa21 in-trogressed region sequence hybrid-izing nearly isogenic line pathogen xanthomonas oryzae pv resistance locus restriction fragment length plymorphism physical size dna fragment xa21 nil random primer sistance locus xa21 polymerase chain re-action polymorphic xa21-linked marker xa21-1inked marker oryza sativa f2 population a21-1inked dna marker rapd818 sequence field gel electrophoresis
