DMCA
Characteristics of a human cell line transformed by DNA from human adenovirus type 5 (1977)
| Citations: | 670 - 14 self |
BibTeX
@MISC{Graham77characteristicsof,
author = {F. L. Graham and J. Smileyt and W. C. Russell and R. Nairn},
title = {Characteristics of a human cell line transformed by DNA from human adenovirus type 5},
year = {1977}
}
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Abstract
Human embryonic kidney cells have been transformed by exposing cells to sheared fragments of adenovirus type 5 DNA. The transformed cells (designated 293 cells) exhibited many of the characteristics of transformation including the elaboration of a virus-specific tumour antigen. Analysis of the polypeptides syn-thesized in the 293 cells by labelling with 35S-methionine and SDS PAGE showed a variable pattern of synthesis, different in a number of respects from that seen in other human cells. On labelling the surface of cells by lactoperoxidase catalysed radio-iodination, the absence of a labelled polypeptide analogous to the 25o K (LETS) glycoprotein was noted. Hybridization of labelled cellular RNA with restriction fragments of adenovirus type 5 DNA indicated transcription of a por-tion of the adenovirus genome at the conventional left hand end.
Keyphrases
human cell line human adenovirus type adenovirus type dna indicated transcription labelled cellular rna conventional left hand end restriction fragment human cell variable pattern labelled polypeptide human embryonic kidney cell sd page virus-specific tumour antigen sheared fragment adenovirus genome transformed cell
