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The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation (1999)
| Venue: | Dev. Biol |
| Citations: | 255 - 7 self |
BibTeX
@ARTICLE{Olsen99thelin-4,
author = {Philip H. Olsen and Victor Ambros},
title = {The lin-4 regulatory RNA controls developmental timing in Caenorhabditis elegans by blocking LIN-14 protein synthesis after the initiation of translation},
journal = {Dev. Biol},
year = {1999},
pages = {671--680}
}
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Abstract
lin-4 encodes a small RNA that is complementary to sequences in the 3 � untranslated region (UTR) of lin-14 mRNA and that acts to developmentally repress the accumulation of LIN-14 protein. This repression is essential for the proper timing of numerous events of Caenorhabditis elegans larval development. We have investigated the mechanism of lin-4 RNA action by examining the fate of lin-14 mRNA in vivo during the time that lin-4 RNA is expressed. Our results indicate that the rate of synthesis of lin-14 mRNA, its state of polyadenylation, its abundance in the cytoplasmic fraction, and its polysomal sedimentation profile do not change in response to the accumulation of lin-4 RNA. Our results indicate that association of lin-4 RNA with the 3 � UTR of lin-14 mRNA permits normal biogenesis of lin-14 mRNA, and normal translational initiation, but inhibits step(s) thereafter, such as translational elongation and/or the release of stable LIN-14 protein. © 1999 Academic Press Key Words: heterochronic genes; translation; C. elegans; lin-4 RNA; polyribosomes.
Keyphrases
lin-14 mrna lin-4 rna lin-4 regulatory rna developmental timing lin-14 protein synthesis caenorhabditis elegans numerous event inhibits step proper timing untranslated region polysomal sedimentation profile academic press key word cytoplasmic fraction stable lin-14 protein heterochronic gene caenorhabditis elegans larval development normal translational initiation translational elongation small rna lin-4 rna action normal biogenesis lin-14 protein
