DMCA
2003. Functional analysis of a divergent system II protein, Ccs1, involved in c-type cytochrome biogenesis
| Citations: | 5 - 1 self |
BibTeX
@MISC{Dreyfuss_2003.functional,
author = {Beth Welty Dreyfuss and Patrice P. Hamel and Stacie S. Nakamoto and Sabeeha Merchant},
title = {2003. Functional analysis of a divergent system II protein, Ccs1, involved in c-type cytochrome biogenesis},
year = {}
}
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Abstract
The Ccs1 gene, encoding a highly divergent novel component of a system II type c-type cytochrome biogenesis pathway, is encoded by the previously defined CCS1 locus in Chlamydomonas reinhardtii. phoA and lacZ� bacterial topological reporters were used to deduce a topological model of the Synechocystis sp. 6803 Ccs1 homologue, CcsB. CcsB, and therefore by analogy Ccs1, possesses a large soluble lumenal domain at its C terminus that is tethered in the thylakoid membrane by three closely spaced transmembrane domains in the N-terminal portion of the protein. Molecular analysis of ccs1 alleles reveals that the entire C-terminal soluble domain is essential for Ccs1 function and that a stromal loop appears to be important in vivo, at least for maintenance of Ccs1. Site-directed mutational analysis reveals that a
Keyphrases
c-type cytochrome biogenesis divergent system ii protein functional analysis analogy ccs1 spaced transmembrane domain stromal loop site-directed mutational analysis reveals molecular analysis ccs1 gene chlamydomonas reinhardtii topological model thylakoid membrane divergent novel component ccs1 locus large soluble lumenal domain n-terminal portion ccs1 allele reveals synechocystis sp ccs1 function ccs1 homologue entire c-terminal soluble domain lacz bacterial topological reporter c-type cytochrome biogenesis pathway system ii
