doi:10.1093/nar/gkp675 Real-time DNA microarray analysis (2009)
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BibTeX
@MISC{Hassibi09doi:10.1093/nar/gkp675real-time,
author = {Arjang Hassibi and Haris Vikalo and José Luis Riechmann and Babak Hassibi},
title = {doi:10.1093/nar/gkp675 Real-time DNA microarray analysis},
year = {2009}
}
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Abstract
We present a quantification method for affinitybased DNA microarrays which is based on the real-time measurements of hybridization kinetics. This method, i.e. real-time DNA microarrays, enhances the detection dynamic range of conventional systems by being impervious to probe saturation in the capturing spots, washing artifacts, microarray spot-to-spot variations, and other signal amplitude-affecting non-idealities. We demonstrate in both theory and practice that the time-constant of target capturing in microarrays, similar to all affinity-based biosensors, is inversely proportional to the concentration of the target analyte, which we subsequently use as the fundamental parameter to estimate the concentration of the analytes. Furthermore, to empirically validate the capabilities of this method in practical applications, we present a FRET-based assay which enables the real-time detection in gene expression DNA microarrays.
Keyphrases
affinity-based biosensors target analyte affinitybased dna microarrays microarray spot-to-spot variation hybridization kinetics signal amplitude-affecting non-idealities detection dynamic range conventional system fret-based assay real-time measurement gene expression dna microarrays fundamental parameter practical application target capturing real-time dna microarrays quantification method real-time detection
