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Doors R, Meerhof L. Size distribution, electronic recognition and counting of human blood monocytes. Blood (1976)
| Citations: | 3 - 0 self |
BibTeX
@MISC{Loos76doorsr,,
author = {Hans Loos and Bep Blok-schut and Rineke Van Doom and Louis Meerhof},
title = {Doors R, Meerhof L. Size distribution, electronic recognition and counting of human blood monocytes. Blood},
year = {1976}
}
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Abstract
During a study on the separation of human blood monocytes from lymphocytes, a method was developed to recognize and count monocytes by electronic means. Lightscattering (Cytograf, Bio/Physics), and changes in electrical resistance (Chan-nelyzer, Coulter) were used to size mono-nuclear leukocytes directly in cell sus-pensions. Both methods revealed a size distribution profile in which two popula-tions of mononuclear leukocytes could be distinguished. The largest cells were virtu-ally eliminated after phagocytosis of iron particles. We confirmed that these cells were monocytes by three different criteria:
