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Polyamine triggering of exocytosis in Paramecium involves an extracellular Ca2+ /(polyvalent cation)-sensing receptor, subplasmalemmal Ca-store mobilization and store-operated Ca2+ -influx via unspecific cation channels (2000)
| Citations: | 14 - 7 self |
BibTeX
@MISC{Klauke00polyaminetriggering,
author = {N. Klauke and M. -p. Blanchard and H. Plattner},
title = {Polyamine triggering of exocytosis in Paramecium involves an extracellular Ca2+ /(polyvalent cation)-sensing receptor, subplasmalemmal Ca-store mobilization and store-operated Ca2+ -influx via unspecific cation channels},
year = {2000}
}
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Abstract
Abstract. The polyamine secretagogue, aminoethyldex-tran (AED), causes a cortical [Ca2+] transient in Para-mecium cells, as analyzed by fluorochrome imag-ing. Our most essential findings are: (i) Cortical Ca2+ signals also occur when AED is applied in presence of the fast Ca2+ chelator, BAPTA. (ii) Extracellular La3+ application causes within seconds a rapid, reversible fluorescence signal whose reversibility can be attributed to a physiological [Ca2+]i transient (while injected La3+ causes a sustained fluorescence signal). (iii) Simply in-creasing [Ca2+]o causes a similar rapid, short-lived [Ca2+]i transient. All these phenomena, (i–iii), are com-patible with activation of an extracellular “Ca2+/ (polyvalent cation)-sensing receptor ” known from some higher eukaryotic systems, where this sensor (responding
Keyphrases
extracellular ca2 polyvalent cation polyamine triggering unspecific cation channel store-operated ca2 influx subplasmalemmal ca-store mobilization para-mecium cell eukaryotic system sustained fluorescence signal physiological ca2 transient fluorochrome imag-ing similar rapid cortical ca2 signal short-lived ca2 transient polyamine secretagogue reversible fluorescence signal essential finding cortical ca2 transient fast ca2 chelator simply in-creasing ca2 extracellular la3 application cause
