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Synchronous exocytosis in Paramecium cells. VI. Ultrastructnral analysis of membrane ~sealing and retrieval (1985)
| Venue: | J. Cell Sci |
| Citations: | 47 - 18 self |
BibTeX
@ARTICLE{Zieseniss85synchronousexocytosis,
author = {Egmont Zieseniss and Helmut Plattner},
title = {Synchronous exocytosis in Paramecium cells. VI. Ultrastructnral analysis of membrane ~sealing and retrieval},
journal = {J. Cell Sci},
year = {1985}
}
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Abstract
ABSTRACT Synchronous exocytosis in Paramecium cells involves the rapid (_<1 s) dephosphorylation of a 65-kD phosphoprotein, which, after a lag phase of ~5 s, is reversed within ~20 s. Exocytosis inhibitors suppress this reaction; stimulatory and inhibitory effects are dose dependent. The dephosphorylation of the 65-kD phosphoprotein occurs only in exocytosiscompetent strains, but not in mutant strains that cannot carry out membrane fusion, or that are devoid of secretory organelles or cannot transport them to the cell membrane. Since under all conditions analyzed the transient dephosphorylation of the 65-kD phosphoprotein strictly parallels the actual amount of exocytosed organelles, this process might be involved in exocytosis performance, perhaps in its initiation. Protein phosphorylation and dephosphorylation processes are now considered to represent a general mechanism for a transient activation of various cell functions (18, 19, 23, 24, 38). Protein phosphorylation accompanies exocytotic activity in
Keyphrases
paramecium cell synchronous exocytosis 65-kd phosphoprotein ultrastructnral analysis membrane sealing abstract synchronous exocytosis cell membrane membrane fusion dephosphorylation process transient dephosphorylation transient activation actual amount exocytosis inhibitor general mechanism protein phosphorylation accompanies exocytotic activity mutant strain inhibitory effect dose dependent secretory organelle exocytosed organelle various cell function protein phosphorylation exocytosis performance exocytosiscompetent strain lag phase
