DMCA
HY: Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs
Cached
Download Links
| Venue: | Squazzo SL, Xu X, Brugmann SA, Goodnough LH, Helms JA, Farnham PJ, Segal E, Chang |
| Citations: | 193 - 3 self |
BibTeX
@INPROCEEDINGS{Rinn_hy:functional,
author = {John L. Rinn and Michael Kertesz and Jordon K. Wang and Sharon L. Squazzo and Xiao Xu and Samantha A. Brugmann and L. Henry Goodnough and Jill A. Helms and Peggy J. Farnham and Eran Segal and Howard Y. Chang},
title = {HY: Functional demarcation of active and silent chromatin domains in human HOX loci by noncoding RNAs},
booktitle = {Squazzo SL, Xu X, Brugmann SA, Goodnough LH, Helms JA, Farnham PJ, Segal E, Chang},
year = {},
pages = {129--1311}
}
Share
 |
 |
 |
 |
||
OpenURL
Abstract
Noncoding RNAs (ncRNA) participate in epigenetic regulation but are poorly understood. Here we characterize the transcriptional landscape of the four human HOX loci at five base pair resolution in 11 anatomic sites and identify 231 HOX ncRNAs that extend known transcribed regions by more than 30 kilobases. HOX ncRNAs are spatially expressed along developmental axes and possess unique sequence motifs, and their expression demarcates broad chromosomal domains of differential histone methylation and RNA polymerase accessibility. We identified a 2.2 kilobase ncRNA residing in the HOXC locus, termed HOTAIR, which represses transcription in trans across 40 kilobases of the HOXD locus. HOTAIR interacts with Polycomb Repressive Complex 2 (PRC2) and is required for PRC2 occupancy and histone H3 lysine-27 trimethylation of HOXD locus. Thus, transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance; these results have broad implications for gene regulation in development and disease states.
Keyphrases
human hox locus silent chromatin domain functional demarcation hoxd locus hox ncrnas polycomb repressive complex developmental ax broad implication base pair resolution chromosomal domain transcribed region rna polymerase accessibility gene regulation broad chromosomal domain transcriptional landscape prc2 occupancy disease state posse unique sequence motif kilobase ncrna hoxc locus anatomic site differential histone methylation histone h3 lysine-27 trimethylation epigenetic regulation
