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... four important research efforts that will improve and accelerate the design cycle for next-generation synthetic gene networks: (1) advancing and expanding the toolkit of available parts and modules, =-=(2)-=- modeling and fine-tuning the behavior of synthetic circuits, (3) developing probes for reliably quantifying state values for synthetic (and natural) biomolecular systems, and (4) creating test platfo...

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...e parts and modules, (2) modeling and fine-tuning the behavior of synthetic circuits, (3) developing probes for reliably quantifying state values for synthetic (and natural) biomolecular systems, and =-=(4)-=- creating test platforms for characterizing component interactions within engineered gene networks, designing gene circuits with increasing complexity, and developing complex circuits for use in highe...

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... the design cycle for next-generation synthetic gene networks: (1) advancing and expanding the toolkit of available parts and modules, (2) modeling and fine-tuning the behavior of synthetic circuits, =-=(3)-=- developing probes for reliably quantifying state values for synthetic (and natural) biomolecular systems, and (4) creating test platforms for characterizing component interactions within engineered g...

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...onstructing synthetic circuits. In the following subsections, we discuss four important research efforts that will improve and accelerate the design cycle for next-generation synthetic gene networks: =-=(1)-=- advancing and expanding the toolkit of available parts and modules, (2) modeling and fine-tuning the behavior of synthetic circuits, (3) developing probes for reliably quantifying state values for sy...

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...ed to T.K.L (timlu@mit.edu). NIH Public Access Author Manuscript Nat Biotechnol. Author manuscript; available in PMC 2010 June 01. Published in final edited form as: Nat Biotechnol. 2009 December ; 27=-=(12)-=-: 1139–1150. doi:10.1038/nbt.1591. N IH -PA Author M anuscript N IH -PA Author M anuscript N IH -PA Author M anuscript networks, and describe some of the more compelling circuits to be developed and a...