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RecBCD enzyme and the repair of double-stranded DNA breaks
- Microbiol. Mol. Rev
, 2008
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A comparison of responses to double-strand breaks between Escherichia coli and Bacillus subtilis reveals different requirements for SOS induction
- J. Bacteriol
, 2008
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These include: This article cites 84 articles, 36 of which can be accessed free at:
In vivo evidence for two active nuclease motifs in the double–strand break repair enzyme RexAB of Lactococcus lactis
- J. Bacteriol
, 2001
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Genetic Requirements for High Constitutive SOS Expression in recA730 Mutants of Escherichia coli
, 2011
"... The RecA protein in its functional state is in complex with single-stranded DNA, i.e., in the form of a RecA filament. In SOS induction, the RecA filament functions as a coprotease, enabling the autodigestion of the LexA repressor. The RecA filament can be formed by different mechanisms, but all of ..."
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The RecA protein in its functional state is in complex with single-stranded DNA, i.e., in the form of a RecA filament. In SOS induction, the RecA filament functions as a coprotease, enabling the autodigestion of the LexA repressor. The RecA filament can be formed by different mechanisms, but all of them require three enzymatic activities essential for the processing of DNA double-stranded ends. These are helicase, 5–3 exonuclease, and RecA loading onto single-stranded DNA (ssDNA). In some mutants, the SOS response can be expressed constitutively during the process of normal DNA metabolism. The RecA730 mutant protein is able to form the RecA filament without the help of RecBCD and RecFOR mediators since it better competes with the single-strand binding (SSB) protein for ssDNA. As a consequence, the recA730 mutants show high constitutive SOS expression. In the study described in this paper, we studied the genetic requirements for constitutive SOS expression in recA730 mutants. Using a -galactosidase assay, we showed that the constitutive SOS response in recA730 mutants exhibits different requirements in different backgrounds. In a wild-type background, the constitutive SOS response is partially dependent on RecBCD function. In a recB1080 background (the recB1080 mutation retains only helicase), constitutive SOS expression is partially dependent on RecBCD helicase function and is strongly dependent on RecJ nuclease. Finally, in a recB-null background, the constitutive SOS expression of the recA730 mutant is
Genetic Evidence for the Requirement of RecA Loading Activity in SOS Induction after UV Irradiation in Escherichia coli
, 2006
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"... on), a e a th cD pe erm ssa ite A m all nz such as conjugation, transductio double-stranded DNA (dsDNA either during DNA replication Michel et al., 1997) or as a resu (for a review, see Friedberg RecBCD enzyme consists of ..."
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on), a e a th cD pe erm ssa ite A m all nz such as conjugation, transductio double-stranded DNA (dsDNA either during DNA replication Michel et al., 1997) or as a resu (for a review, see Friedberg RecBCD enzyme consists of
Effects of recJ, recQ, and recFOR Mutations on Recombination in Nuclease-Deficient recB recD Double Mutants of Escherichia coli
, 2004
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This article was originally published in a journal published by Elsevier, and the attached copy is provided by Elsevier for the author’s benefit and for the benefit of the author’s institution, for non-commercial research and educational use including without limitation use in instruction at your institution, sending it to specific colleagues that you know, and providing a copy to your institution’s administrator. All other uses, reproduction and distribution, including without limitation commercial reprints, selling or licensing copies or access, or posting on open internet sites, your personal or institution’s website or repository, are prohibited. For exceptions, permission may be sought for such use through Elsevier’s permissions site at:
