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Application of FTIR spectroscopy for a rapid determination of some hydrolytic enzymes activity on sea buckthorn substrate (2010)

by C Adina, F Florinela, T Abdelmoumen, S Carmen
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Research Article Investigation of Bacterial Cellulose Biosynthesis Mechanism in Gluconoacetobacter hansenii

by Bhavna V. Mohite, Satish V. Patil
"... License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The present study explores the mechanism of cellulose biosynthesis in Gluconoacetobacter hansenii. The cellulose synthase enzyme was purified as membrane fraction and ..."
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License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The present study explores the mechanism of cellulose biosynthesis in Gluconoacetobacter hansenii. The cellulose synthase enzyme was purified as membrane fraction and solubilized by treatment with 0.1 % digitonin. The enzyme was separated by native-gel electrophoresis and
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...nge molecular weight marker (lane 2). (b) Glycoprotein band of cellulose synthase on native gel. was considered for the identification of cellulase hydrolytic activity and the rate of glucose release =-=[26, 27]-=-. 3.3. SDS PAGE of Purified Cellulose Synthase. The bands detected by Coomassie brilliant blue staining was compared with high range molecular weight marker. Two distinct bands were observed correspon...

thermo-sensitive polymers present in aqueous

by Subbarayalu Ramalakshmi A, Chien Wei Ooi A
"... Colorimetric quantification of sucrose in presence of ..."
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Colorimetric quantification of sucrose in presence of
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...ntration of the phase-forming components is essential for tie-line construction in ATPS study. Concentration of sugars in the phases can be determined with the help of chromatographic [8,9], physical =-=[10,11]-=- and colorimetric methods [1–3,12]. Among all methods of carbohydrate analysis, colorimetric methods have the advantages such as less time consumption, inexpensive, high throughput and easy workflow. ...

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