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Conflicting mitochondrial and nuclear phylogenies for the widely disjunct Emys (Testudines: Emydidae) species complex, and what they tell us about biogeography and hybridization. (2009)

by P Q Spinks, H B Shaffer
Venue:Syst. Biol.
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When trees grow too long: investigating the causes of highly inaccurate Bayesian branch-length estimates. Syst. Biol

by Jeremy M. Brown, Shannon M. Hedtke, Alan R. Lemmon, Emily, Moriarty Lemmon , 2010
"... Abstract.—A surprising number of recent Bayesian phylogenetic analyses contain branch-length estimates that are several orders of magnitude longer than corresponding maximum-likelihood estimates. The levels of divergence implied by such branch lengths are unreasonable for studies using biological da ..."
Abstract - Cited by 28 (1 self) - Add to MetaCart
Abstract.—A surprising number of recent Bayesian phylogenetic analyses contain branch-length estimates that are several orders of magnitude longer than corresponding maximum-likelihood estimates. The levels of divergence implied by such branch lengths are unreasonable for studies using biological data and are known to be false for studies using simulated data. We conducted additional Bayesian analyses and studied approximate-posterior surfaces to investigate the causes underlying these large errors. We manipulated the starting parameter values of the Markov chain Monte Carlo (MCMC) analyses, the moves used by the MCMC analyses, and the prior-probability distribution on branch lengths. We demonstrate that inaccurate branch-length estimates result from either 1) poor mixing of MCMC chains or 2) posterior distributions with excessive weight at long tree lengths. Both effects are caused by a rapid increase in the volume of

Deep genealogical lineages in the widely distributed African helmeted terrapin: evidence from mitochondrial and nuclear DNA (Testudines: Pelomedusidae: Pelomedusa subrufa). Mol Phylogenet Evol 56:428–440.

by Mario Vargas-Ramírez , Miguel Vences , William R Branch , Savel R Daniels , Frank Glaw , Margaretha D Hofmeyr , Gerald Kuchling , Jérôme Maran , Theodore J Papenfuss , Pavel Široký , David R Vieites , Uwe Fritz , 2010
"... a b s t r a c t We investigated the phylogeographic differentiation of the widely distributed African helmeted terrapin Pelomedusa subrufa based on 1503 base pairs of mitochondrial DNA (partial cyt b and ND4 genes with adjacent tRNAs) and 1937 bp of nuclear DNA (partial Rag1, Rag2, R35 genes). Cong ..."
Abstract - Cited by 4 (4 self) - Add to MetaCart
a b s t r a c t We investigated the phylogeographic differentiation of the widely distributed African helmeted terrapin Pelomedusa subrufa based on 1503 base pairs of mitochondrial DNA (partial cyt b and ND4 genes with adjacent tRNAs) and 1937 bp of nuclear DNA (partial Rag1, Rag2, R35 genes). Congruent among different analyses, nine strongly divergent mitochondrial clades were found, representing three major geographical groupings: (1) A northern group which includes clades I from Cameroon, II from Ghana and Ivory Coast, III from Benin, Burkina Faso and Niger, IV from the Central African Republic, and V from Kenya, (2) a northeastern group consisting of clades VI from Somalia, and VII from Saudi Arabia and Yemen, and (3) a southern group comprising clade VIII from Botswana, the Democratic Republic of Congo, Madagascar and Malawi, and clade IX from South Africa. Malagasy and continental African populations were not clearly differentiated, indicating very recent arrival or introduction of Pelomedusa in Madagascar. The southern group was in some phylogenetic analyses sister to Pelusios, rendering Pelomedusa paraphyletic with respect to that genus. However, using partitioned Bayesian analyses and sequence data of the three nuclear genes, Pelomedusa was monophyletic, suggesting that its mitochondrial paraphyly is due to either ancient introgressive hybridization or phylogenetic noise. Otherwise, nuclear sequence data recovered a lower level of divergence, but corroborated the general differentiation pattern of Pelomedusa as revealed by mtDNA. This, and the depth of the divergences between clades, indicates ancient differentiation. The divergences observed fall within, and in part exceed considerably, the differentiation typically occurring among chelonian species. To test whether Pelomedusa is best considered a single species composed of deep genealogical lineages, or a complex of up to nine distinct species, we suggest a future taxonomic revision that should (1) extend the geographical sampling of molecular data, specifically focusing on contact zones and the possible sympatric occurrence of lineages without admixture, and (2) evaluate the morphology of the various genealogical lineages using the type specimens or topotypical material of the numerous junior synonyms of P. subrufa.
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...netic noise. This view is corroborated by plotting transitions and transversions against corrected pairwise divergences, which indicates mutational saturation mainly at the third codon positions. Yet, explorative unpartitioned analyses of cyt b and ND4 sequences placed P. sinuatus as sister to the two southern Pelomedusa lineages, also when then the third codon position was excluded (not shown). Alternatively to saturation, the observed paraphyly could be the result of ancient hybridization with introgression, as recently suggested for North American and Asian terrapins (Actinemys, Emydoidea: Spinks and Shaffer, 2009, but see Wiens et al., 2010; Cuora: Spinks and Shaffer, 2007). It is obvious that future efforts should be undertaken to analyze the phylogenetic relationships of all Pelusios species and Pelomedusa to gain a better understanding of the relation-ineages in the widely distributed African helmeted terrapin: Evidence from ufa). Mol. Phylogenet. Evol. (2010), doi:10.1016/j.ympev.2010.03.019 8 M. Vargas-Ramírez et al. / Molecular Phylogenetics and Evolution xxx (2010) xxx–xxx ARTICLE IN PRESSships and of the evolutionary history of both pelomedusid genera.4.3. Vicariance of the Arabian clade and p...

Phylogeny estimation of the radiation of western North American chipmunks (Tamias) in the face of introgression using reproductive protein genes. Syst. Biol

by Noah Reid, John R. Demboski, Jack Sullivan
"... Abstract.—The causes and consequences of rapid radiations are major unresolved issues in evolutionary biology. This is in part because phylogeny estimation is confounded by processes such as stochastic lineage sorting and hybridization. Because these processes are expected to be heterogeneous across ..."
Abstract - Cited by 4 (0 self) - Add to MetaCart
Abstract.—The causes and consequences of rapid radiations are major unresolved issues in evolutionary biology. This is in part because phylogeny estimation is confounded by processes such as stochastic lineage sorting and hybridization. Because these processes are expected to be heterogeneous across the genome, comparison among marker classes may provide a means of disentangling these elements. Here we use introns from nuclear-encoded reproductive protein genes expected to be resistant to introgression to estimate the phylogeny of the western chipmunks (Tamias: subgenus: Neotamias), a rapid radiation that has experienced introgressive hybridization of mitochondrial DNA (mtDNA). We analyze the nuclear loci using coalescent-based species-tree estimation methods and concatenation to estimate a species tree and we use parametric bootstraps and coalescent simulations to differentiate between phylogenetic error, coalescent stochasticity and introgressive hybridization. Results indicate that the mtDNA gene tree reflects several introgression events that have occurred between taxa of varying levels of divergence and at different time points in the tree. T. panamintinus and T. speciosus appear to be fixed for ancient mitochondrial introgressions from T. minimus. A southern Rocky Mountains clade appears well sorted (i.e.,

To name or not to name: Criteria to promote economy of change in

by Miguel Vences, Juan M. Guayasamin, Aurélien Miralles, Ignacio De, La Riva
"... www.mapress.com/zootaxa/ ..."
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www.mapress.com/zootaxa/
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...Emydoidea. The CRITERIA FOR NAMING & STABILITY IN LINNAEAN CLASSIFICATIONS Zootaxa 3636 (2) © 2013 Magnolia Press · 231concerned species had previously been merged in a single, inclusive genus Emys (=-=Spinks & Shaffer 2009-=-), but the monophyly of such an inclusive Emys was contradicted by a subset of possible gene trees (Wiens et al. 2010) and the respective clade stability thus compromised. A fully resolved phylogeneti...

ORIGINAL ARTICLE

by Ss Suni, Dm Gordon , 2009
"... www.nature.com/hdy Fine-scale genetic structure and dispersal distance in the harvester ant Pogonomyrmex barbatus ..."
Abstract - Cited by 1 (0 self) - Add to MetaCart
www.nature.com/hdy Fine-scale genetic structure and dispersal distance in the harvester ant Pogonomyrmex barbatus

Coalescent species delimitation in milksnakes (genus Lampropeltis) and impacts on phylogenetic comparative analyses

by Sara Ruane, Robert W. Bryson, R. Alexander Pyron, Frank, T. Burbrink - Systematic Biology , 2014
"... Abstract.—Both gene-tree discordance and unrecognized diversity are sources of error for accurate estimation of species trees, and can affect downstream diversification analyses by obscuring the correct number of nodes, their density, and the lengths of the branches subtending them. Although the the ..."
Abstract - Cited by 1 (0 self) - Add to MetaCart
Abstract.—Both gene-tree discordance and unrecognized diversity are sources of error for accurate estimation of species trees, and can affect downstream diversification analyses by obscuring the correct number of nodes, their density, and the lengths of the branches subtending them. Although the theoretical impact of gene-tree discordance on evolutionary analyses has been examined previously, the effect of unsampled and cryptic diversity has not. Here, we examine how delimitation of previously unrecognized diversity in the milksnake (Lampropeltis triangulum) and use of a species-tree approach affects both estimation of the Lampropeltis phylogeny and comparative analyses with respect to the timing of diversification. Coalescent species delimitation indicates that L. triangulum is not monophyletic and that there are multiple species of milksnake, which increases the known species diversity in the genus Lampropeltis by 40%. Both genealogical and temporal discordance occurs between gene trees and the species tree, with evidence that mitochondrial DNA (mtDNA)

Author's personal copy Deep genealogical lineages in the widely distributed African helmeted terrapin: Evidence from mitochondrial and nuclear DNA (Testudines: Pelomedusidae: Pelomedusa subrufa)

by Mario Vargas-Ramírez , Miguel Vences , William R Branch , Savel R Daniels , Frank Glaw , Margaretha D Hofmeyr , Gerald Kuchling , Jérôme Maran , Theodore J Papenfuss , Pavel Široký , David R Vieites , Uwe Fritz , 2010
"... a b s t r a c t We investigated the phylogeographic differentiation of the widely distributed African helmeted terrapin Pelomedusa subrufa based on 1503 base pairs of mitochondrial DNA (partial cyt b and ND4 genes with adjacent tRNAs) and 1937 bp of nuclear DNA (partial Rag1, Rag2, R35 genes). Cong ..."
Abstract - Add to MetaCart
a b s t r a c t We investigated the phylogeographic differentiation of the widely distributed African helmeted terrapin Pelomedusa subrufa based on 1503 base pairs of mitochondrial DNA (partial cyt b and ND4 genes with adjacent tRNAs) and 1937 bp of nuclear DNA (partial Rag1, Rag2, R35 genes). Congruent among different analyses, nine strongly divergent mitochondrial clades were found, representing three major geographical groupings: (1) A northern group which includes clades I from Cameroon, II from Ghana and Ivory Coast, III from Benin, Burkina Faso and Niger, IV from the Central African Republic, and V from Kenya, (2) a northeastern group consisting of clades VI from Somalia, and VII from Saudi Arabia and Yemen, and (3) a southern group comprising clade VIII from Botswana, the Democratic Republic of Congo, Madagascar and Malawi, and clade IX from South Africa. Malagasy and continental African populations were not clearly differentiated, indicating very recent arrival or introduction of Pelomedusa in Madagascar. The southern group was in some phylogenetic analyses sister to Pelusios, rendering Pelomedusa paraphyletic with respect to that genus. However, using partitioned Bayesian analyses and sequence data of the three nuclear genes, Pelomedusa was monophyletic, suggesting that its mitochondrial paraphyly is due to either ancient introgressive hybridization or phylogenetic noise. Otherwise, nuclear sequence data recovered a lower level of divergence, but corroborated the general differentiation pattern of Pelomedusa as revealed by mtDNA. This, and the depth of the divergences between clades, indicates ancient differentiation. The divergences observed fall within, and in part exceed considerably, the differentiation typically occurring among chelonian species. To test whether Pelomedusa is best considered a single species composed of deep genealogical lineages, or a complex of up to nine distinct species, we suggest a future taxonomic revision that should (1) extend the geographical sampling of molecular data, specifically focusing on contact zones and the possible sympatric occurrence of lineages without admixture, and (2) evaluate the morphology of the various genealogical lineages using the type specimens or topotypical material of the numerous junior synonyms of P. subrufa.
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...netic noise. This view is corroborated by plotting transitions and transversions against corrected pairwise divergences, which indicates mutational saturation mainly at the third codon positions. Yet, explorative unpartitioned analyses of cyt b and ND4 sequences placed P. sinuatus as sister to the two southern Pelomedusa lineages, also when then the third codon position was excluded (not shown). Alternatively to saturation, the observed paraphyly could be the result of ancient hybridization with introgression, as recently suggested for North American and Asian terrapins (Actinemys, Emydoidea: Spinks and Shaffer, 2009, but see Wiens et al., 2010; Cuora: Spinks and Shaffer, 2007). It is obvious that future efforts should be undertaken to analyze the phylogenetic relationships of all Pelusios species and Pelomedusa to gain a better understanding of the relation434 M. Vargas-Ramírez et al. / Molecular Phylogenetics and Evolution 56 (2010) 428–440 Author's personal copy ships and of the evolutionary history of both pelomedusid genera. 4.3. Vicariance of the Arabian clade and possible introduction of Pelomedusa to Madagascar While the pronounced mitochondrial divergence of the Arabian clade VII and the Somali c...

Molecular phylogenetics of the mud and musk turtle family Kinosternidae

by John B Iverson , Minh Le , Colleen Ingram
"... a b s t r a c t The turtle family Kinosternidae comprises 25 living species of mud and musk turtles confined to the New World. Previous attempts to reconstruct a phylogenetic history of the group have employed morphological, isozyme, and limited mitochondrial DNA sequence data, but have not been su ..."
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a b s t r a c t The turtle family Kinosternidae comprises 25 living species of mud and musk turtles confined to the New World. Previous attempts to reconstruct a phylogenetic history of the group have employed morphological, isozyme, and limited mitochondrial DNA sequence data, but have not been successful in producing a well-resolved phylogeny. With tissues from every recognized species and most subspecies, we sequenced three mitochondrial (cyt b, 12S, 16S) and three nuclear markers (C-mos, RAG1, RAG2). Our analyses revealed the existence of three well-resolved clades within the Kinosterninae (aged >22 mya), only two of which have been named: Sternotherus and Kinosternon. We here describe the third clade as a new genus. The evolutionary relationships among most species were well resolved, although those belonging to the K. scorpioides species group will require more extensive geographic and genetic sampling. Divergence time estimates and ancestral area reconstructions permitted the development of the first rigorous hypothesis of the zoogeographic history of the group, including support for three separate dispersals into South America, at least two of which preceded the closure of the Panamanian portal.
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...are indicated by circled C. P + Q = Pliocene + Quaternary. J.B. Iverson et al. / Molecular Phylogenetics and Evolution 69 (2013) 929–939 9334. Discussion 4.1. Taxonomic implications Our analysis confirmed the monophyly of the two previously recognized clades within the family Kinosternidae, one including the genera Staurotypus and Claudius, and the other including Sternotherus and Kinosternon. The fossil record dates the divergence of these two clades at P54 mya (see Section 2.3). Given (1) that the other North American turtle subfamilies date from only 34 mya (emydids) to 52 mya (geoemydids; Spinks and Shaffer, 2009), (2) that other speciose cryptodiran turtle families date from ca. 52 to 70 mya (Testudinidae and Geoemydidae), 60– 90 mya (Emydidae), and 100–129 mya (Trionychidae) (Lourenço et al., 2012; Spinks and Shaffer, 2009; Wang et al., 2012), and (3) that the two subfamilies Staurotypinae and Kinosterninae are unambiguously distinct morphologically (Hutchison, 1991) and in their sex-determining mechanisms (Fig. 1; Ewert et al., 2004), we follow Bickham and Carr (1983; among others) in recognizing these two clades as separate families (Staurotypidae and Kinosternidae; Table 3). Our analysis also reso...

DOI:10.1093/sysbio/syt099 Coalescent Species Delimitation in Milksnakes (Genus Lampropeltis) and Impacts on Phylogenetic Comparative Analyses

by Sara Ruane, Robert W. Bryson, R. Alexander Pyron, Frank, T. Burbrink
"... Abstract.—Both gene-tree discordance and unrecognized diversity are sources of error for accurate estimation of species trees, and can affect downstream diversification analyses by obscuring the correct number of nodes, their density, and the lengths of the branches subtending them. Although the the ..."
Abstract - Add to MetaCart
Abstract.—Both gene-tree discordance and unrecognized diversity are sources of error for accurate estimation of species trees, and can affect downstream diversification analyses by obscuring the correct number of nodes, their density, and the lengths of the branches subtending them. Although the theoretical impact of gene-tree discordance on evolutionary analyses has been examined previously, the effect of unsampled and cryptic diversity has not. Here, we examine how delimitation of previously unrecognized diversity in the milksnake (Lampropeltis triangulum) and use of a species-tree approach affects both estimation of the Lampropeltis phylogeny and comparative analyses with respect to the timing of diversification. Coalescent species delimitation indicates that L. triangulum is not monophyletic and that there are multiple species of milksnake, which increases the known species diversity in the genus Lampropeltis by 40%. Both genealogical and temporal discordance occurs between gene trees and the species tree, with evidence that mitochondrial DNA (mtDNA)

Competing generic concepts for Blanding’s, Pacific and European pond turtles

by Emys)—which Is Best, Uwe Fritz, Christian Schmidt, Carl H. Ernst
"... We review competing taxonomic classifications and hypotheses for the phylogeny of emydine turtles. The formerly recognized genus Clemmys sensu lato clearly is paraphyletic. Two of its former species, now Glyptemys insculpta and G. muhlenbergii, constitute a well-supported basal clade within the Emyd ..."
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We review competing taxonomic classifications and hypotheses for the phylogeny of emydine turtles. The formerly recognized genus Clemmys sensu lato clearly is paraphyletic. Two of its former species, now Glyptemys insculpta and G. muhlenbergii, constitute a well-supported basal clade within the Emydinae. However, the phylogenetic position of the other two species traditionally placed in Clemmys remains controversial. Mitochondrial data suggest a clade embracing Actinemys (formerly Clemmys) marmorata, Emydoidea and Emys and as its sister either another clade (Clemmys guttata + Terrapene) or Terrapene alone. In contrast, nuclear genomic data yield conflicting results, depending on which genes are used. Either Clemmys guttata is revealed as sister to ((Emydoidea + Emys) + Actinemys) + Terrapene or Clemmys guttata is sister to Actinemys marmorata and these two species together are the sister group of (Emydoidea + Emys); Terrapene appears then as sister to (Actinemys marmorata + Clemmys guttata) + (Emydoidea + Emys). The contradictory branching patterns depending from the selected loci are suggestive of lineage sorting problems. Ignoring the unclear phylogenetic position of Actinemys marmorata, one recently proposed classification scheme placed Actinemys marmorata, Emydoidea blandingii, Emys orbicularis, and Emys trinacris in one genus (Emys), while another classification scheme treats Actinemys, Emydoidea, and Emys as distinct genera. The inclusion of Actinemys in the same taxon as Emydoidea + Emys is unacceptable under a phylogenetic classification framework because there is evidence for the non-monophyly of
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...orbicularis shown as polytomy). 44 · Zootaxa 2791 © 2011 Magnolia Press FRITZ ET AL.Later molecular studies using other mitochondrial genes and nuclear loci (Lenk et al. 1999; Feldman & Parham 2002; =-=Spinks & Shaffer 2009-=-; Wiens et al. 2010) corroborated the paraphyly of Clemmys. In the course of a phylogeographic study of Emys orbicularis based on cyt b sequences and using all other emydine genera as outgroups, Lenk ...

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