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967
Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics
- Mol. Cell. Proteomics
, 2002
"... The abbreviations used are: SILAC: Stable isotope labeling by amino acids in cell culture, 2DE: two dimensional (isoelectric focusing/SDS-PAGE) gel electrophoresis: ICATTM: isotope-coded affinity tag; MS: mass spectrometry; MALDI-TOF: matrix assisted laser desorption ionization-time of flight; PMF: ..."
Abstract
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Cited by 595 (23 self)
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have shown great promise for the simultaneous and automated identification and quantitation of complex protein mixtures. Here we describe a method, termed SILAC for Stable Isotope Labeling by Amino acids in Cell culture, for the in vivo incorporation of specific amino acids into all mammalian proteins
o radioactive iodine treatment. n
"... o the treatment of mutations were identified in two and six of these 23 fatal J-Y Lu and others NRAS/GNAS mutations in Follicular thyroid carcinoma ID: 15-0067; January 2016 DOI: 10.1530/EDM-15-0067confirmed by direct Sanger sequencing. The patient’sBackground We report a 79-year-old man with follic ..."
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or gain-of-function mutation resulting i Gs-alpha protein and downstream cAMP cascade, independent of TSH signaling, causing a thyroid nodules. † NRAS p.Q61K mutations with GNAS p.R201H mutations are known for a good radioactive response. † Further exploration of the GNAS-activating pathway may provide
The determination of plasma volume in man with radioactive chromic chloride
- J Clin Invest
"... Radioactive chromium has been found to tag red blood cells and plasma proteins (1). The anionic form, Na2Cr5104, labels the red blood cells, while the cationic hexavalent chromic chloride is firmly bound by the plasma proteins and is not taken up by the red cells. The affinity of the erythrocytes fo ..."
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Cited by 3 (0 self)
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Radioactive chromium has been found to tag red blood cells and plasma proteins (1). The anionic form, Na2Cr5104, labels the red blood cells, while the cationic hexavalent chromic chloride is firmly bound by the plasma proteins and is not taken up by the red cells. The affinity of the erythrocytes
HEMOGLOBIN LABELED BY RADIOACTIVE LYSINE ERYTHROCYTE LIFE CYCLE*,$,§
"... This paper reports on the utilization of the tagged epsilon carbon of d/-lysine by a dog rendered both anemic and hypoproteinemic by repeated bleeding and a diet low in protein. The experiment extended over a period of 234 days, a time sufficient to indicate an erythrocyte life span of at least 115 ..."
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This paper reports on the utilization of the tagged epsilon carbon of d/-lysine by a dog rendered both anemic and hypoproteinemic by repeated bleeding and a diet low in protein. The experiment extended over a period of 234 days, a time sufficient to indicate an erythrocyte life span of at least 115
Radioactive labeling of mitochondrial translation products in cultured cells
- Methods Mol. Biol
, 2012
"... The mammalian mitochondrial genome contains 37 genes, 13 of which encode polypeptide subunits in the enzyme complexes of the oxidative phosphorylation system. The other genes encode the rRNAs and tRNAs necessary for their translation. The mitochondrial translation machinery is located in the mitocho ..."
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Cited by 5 (4 self)
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in the mitochon-drial matrix, and is exclusively dedicated to the synthesis of these 13 enzyme subunits. Mitochondrial disease in humans is often associated with defects in mitochondrial translation. This can manifest as a global decrease in the rate of mitochondrial protein synthesis, a decrease in the synthesis
STUDIES WITH RADIOACTIVE DI-AZO DYES. II. THE SYNTHESIS AND PROPERTIES OF RADIOACTIVE DI-BROM TRYPAN BLUE AND RADIOACTIVE DI-BROM EVANS BLUE'
"... Investigations from these laboratories ( 1, 2) deal with the accumulation of radioactive di-brom dyes in abscesses and tumors. The radio-dyes are also useful for studies of capillary permeability in so far as their permeation from blood into lymph, too slight to be detected visually in the normal an ..."
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animal, may be quantitatively measured on samples of lymph (3). Dyes of this group make a bond with plasma protein (4), becoming preferentially associated with albumin, and they therefore constitute a radioactive "tracer " for protein under certain experimental conditions. In view
Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zyrnogen granules. or. Cell Biol
, 1967
"... It has been established by electron microscopic radioautography of guinea pig pancreatic exocrine cells (Caro and Palade, 1964) that secretory proteins are transported from the elements of the rough-surfaced endoplasmic reticulum (ER) to condensing vacuoles of the Golgi complex possibly via small ve ..."
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Cited by 91 (4 self)
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. Such slices remain viable for 3 hr and incur minimal structural damage in this time. Their secretory proteins can be labeled with radioactive amino acids in short, well defined pulses which, followed by cell fractionation, makes possible a kinetic analysis of transport. To determine the kinetics of transport
CYTOPLASMIC SYNTHESIS OF NUCLEAR PROTEINS Kinetics of Accumulation of Radioactive Proteins in Various Cell Fractions after Brief Pulses
"... The synthesis of cytoplasmic and nuclear proteins has been studied in HeLa cells by examining the amount of radioactive protein appearing in the various subcellular fractions after labeling for brief periods. Due to the rapid equilibration of the amino acid pool, the total radioactivity in cytoplasm ..."
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The synthesis of cytoplasmic and nuclear proteins has been studied in HeLa cells by examining the amount of radioactive protein appearing in the various subcellular fractions after labeling for brief periods. Due to the rapid equilibration of the amino acid pool, the total radioactivity
Circulating red cell volume measured simultaneously by the radioactive iron and dye methods
- J. Clin. Invest
, 1946
"... The measurement of the circulating red cell volume is of considerable value in the study of the circulation in both the normal state and in ex-perimentally induced abnormal circulatory states, and in disease. Modern modifications employing Evans Blue (T-1824) (1 to 3) of the original dye method of K ..."
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Cited by 15 (1 self)
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of blood samples are corrected for variations in water content as determined by serum protein measure-ments, should increase the applicability of the technique. There is no general agreement among authors that the dye plasma technique measures either the total or circulating red cell volume. The opinion
THE USE OF RADIOACTIVE LYSINE IN STUDIES OF PROTEIN METABOLISM SYNTHESIS AND UTILIZATION " OF PLASMA PROTEINS*,$,§
"... We have long been interested in protein metabolism, blood protein production and exchange, body protein needs as sustained by plasma protein or hemoglobin given parenterally, and the labile exchange between reserve protein stores and circulating plasma proteins. This adds up to a dynamic equilibrium ..."
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We have long been interested in protein metabolism, blood protein production and exchange, body protein needs as sustained by plasma protein or hemoglobin given parenterally, and the labile exchange between reserve protein stores and circulating plasma proteins. This adds up to a dynamic
Results 1 - 10
of
967
