Results 11 - 20 of 24

unknown title

by Samantha Miller, Wendy Bartlett, Subramanian Chandrasekaran, Sally Simpson, Michelle Edwards, Ian R. Booth
"... The major structural features of the Escherichia coli MscS mechanosensitive channel protein have been explored using alkaline phosphatase (PhoA) fusions, precise deletions and site-directed mutations. PhoA protein fusion data, combined with the positive-inside rule, strongly support a model in which ..."
Abstract - Add to MetaCart
The major structural features of the Escherichia coli MscS mechanosensitive channel protein have been explored using alkaline phosphatase (PhoA) fusions, precise deletions and site-directed mutations. PhoA protein fusion data, combined with the positive-inside rule, strongly support a model

Role of the Escherichia coli Hfq protein in GcvB regulation of oppA and dppA mRNAs

by Sarah C. Pulvermacher, Lorraine T. Stauffer, George V. Stauffer, George V. Stauffer - Microbiology
"... The gcvB gene encodes a small non-translated RNA (referred to as GcvB) that regulates oppA and dppA, two genes that encode periplasmic binding proteins for the oligopeptide and dipeptide transport systems. Hfq, an RNA chaperone protein, binds many small RNAs and is required for the small RNAs to reg ..."
Abstract - Cited by 6 (2 self) - Add to MetaCart
to regulate expression of their respective target genes. We showed that repression by GcvB of dppA: : lacZ and oppA: : phoA translational fusions is dependent upon Hfq. Double mutations in gcvB and hfq yielded similar expression levels of dppA: : lacZ and oppA: : phoA compared with gcvB or hfq single

Essential residues in the chromate transporter ChrA of Pseudomonas aeruginosa

by Selene Aguilera A, Ma. Esther Aguilar A, Martha P. Cha¤vez A, Joel E. Lo¤pez-meza B , 2004
"... The chrA gene of Pseudomonas aeruginosa plasmid pUM505 encodes the hydrophobic protein ChrA, which confers resistance to chromate by the energy-dependent efflux of chromate ions. Chromate-sensitive mutants were isolated by in vivo random mutagenesis. Transport experiments with cell suspensions of se ..."
Abstract - Add to MetaCart
of selected mutants showed that 51CrO234 extrusion was drastically lowered as compared to suspensions of the strain with the wild-type plasmid, confirming that the mutations affected a chromate efflux system. DNA sequence analysis showed that most point mutations affected amino acids clustered in the N

unknown title

by unknown authors
"... Three distinct systems (I, II, and III) for catalysis of heme attachment to c-type apocytochromes are known. The CcsA and Ccs1 proteins are required in system II for the assembly of bacterial and plastid cytochromes c. A tryptophan-rich signature motif (WWD), also occurring in CcmC and CcmF found in ..."
Abstract - Add to MetaCart
Three distinct systems (I, II, and III) for catalysis of heme attachment to c-type apocytochromes are known. The CcsA and Ccs1 proteins are required in system II for the assembly of bacterial and plastid cytochromes c. A tryptophan-rich signature motif (WWD), also occurring in CcmC and CcmF found

Tropical Biomedicine 28(1): 21–31 (2011) Simultaneous detection of methicillin-resistant Staphylococcus aureus, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae and

by Pseudomonas Aeruginosa Multiplex Pcr , 2010
"... Abstract. A PCR-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed. Six pairs of selected primers targeting femA (132 bp) and mecA (310 bp) of methicillin-resistant Staphylococcus aureus, gltA (722 bp) of Acinetobacter ba ..."
Abstract - Add to MetaCart
Abstract. A PCR-based assay that can simultaneously detect and differentiate five different types of nosocomial bacterial pathogens was developed. Six pairs of selected primers targeting femA (132 bp) and mecA (310 bp) of methicillin-resistant Staphylococcus aureus, gltA (722 bp) of Acinetobacter

The VirB4 ATPase of Agrobacterium tumefaciens Is a Cytoplasmic Membrane Protein Exposed at the

by Periplasmic Surface, Tu Anh, T. Dang, Peter, J. Christie , 1996
"... The VirB4 ATPase of Agrobacterium tumefaciens, a putative component of the T-complex transport apparatus, associates with the cytoplasmic membrane independently of other products of the Ti plasmid. VirB4 was resistant to extraction from membranes of wild-type strain A348 or a Ti-plasmidless strain e ..."
Abstract - Add to MetaCart
The VirB4 ATPase of Agrobacterium tumefaciens, a putative component of the T-complex transport apparatus, associates with the cytoplasmic membrane independently of other products of the Ti plasmid. VirB4 was resistant to extraction from membranes of wild-type strain A348 or a Ti-plasmidless strain

The synthetic Plotkin powerdomain

by Wesley Phoa, Paul Taylor , 1990
"... Plotkin [1976] introduced a powerdomain construction on domains in order to give semantics to a non-deterministic binary choice constructor, and later [1979] characterised it as the free semilattice. Smyth [1983] and Winskel [1985] showed that it could be interpreted in terms of modal predicate tran ..."
Abstract - Cited by 2 (0 self) - Add to MetaCart
compare them with the classical approach. Important Note: Much of the credit for the work in this paper is due to Wesley Phoa and Martin Hyland, but I take the blame for its presentation. Comments on it are most welcome. When it is finished it will be submitted as a joint paper with Wesley Phoa

Full Abstraction and Universality via Realisability

by Michael Marz, Alexander Rohr, Thomas Streicher, D- Darmstadt , 1999
"... ion and Universality via Realisability Michael Marz marz@mathematik.tu-darmstadt.de Alexander Rohr y rohr@mathematik.tu-darmstadt.de Thomas Streicher streicher@mathematik.tu-darmstadt.de Technische Universitat Darmstadt Fachbereich Mathematik Schlogartenstrae 7, D-64289 Darmstadt Abstra ..."
Abstract - Cited by 4 (0 self) - Add to MetaCart
Abstract We construct fully abstract realisability models of PCF. In particular, we prove a variant of the Longley--Phoa Conjecture by showing that the realisability model over an untyped --calculus with arithmetic is fully abstract for PCF. Further we consider the extension of our results to a general

CHAPTER IV Membrane topology of the lactococcal bacteriocin ABC transporter protein LcnC; involvement of LcnC in lactococcin A maturation

by unknown authors
"... SUMMARY Many non-lantibiotic bacteriocins of lactic acid bacteria are produced as precursors with N-terminal leader peptides different from those present in preproteins exported by the general sec-dependent (Type II) secretion pathway. These bacteriocins utilize a dedicated (Type I) secretion system ..."
Abstract - Add to MetaCart
accessory proteins of Type I secretion systems. This paper shows that the N-terminal part of LcnC is involved in the processing of the precursor of LcnA. By making translational fusions of LcnC to the reporter proteins ß-galactosidase (LacZ) and alkaline phosphatase (PhoA*) it was shown that both the N

doi: 10.1038/nature08559 SUPPLEMENTARY INFORMATION Supplementary Materials and methods Genetic constructs:

by Construction Of Prophoa-his
"... proPhoA(1-62)-His for purification purposes: proPhoA-His6: The wild type proPhoA gene (Expasy access code: P00634) was isolated by PCR from the chromosome of E.coli JM109(DE3) using X560 (5 ’ GGG AAT TCC ATA TGA AAC AAA GCA CTA TTG CA 3’) that inserts an Nde I site as forward primer and X561 (5 ’ GA ..."
Abstract - Add to MetaCart
proPhoA(1-62)-His for purification purposes: proPhoA-His6: The wild type proPhoA gene (Expasy access code: P00634) was isolated by PCR from the chromosome of E.coli JM109(DE3) using X560 (5 ’ GGG AAT TCC ATA TGA AAC AAA GCA CTA TTG CA 3’) that inserts an Nde I site as forward primer and X561 (5
Results 11 - 20 of 24