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High-Throughput Method for Detecting
, 2004
"... These include: This article cites 38 articles, 25 of which can be accessed free at: ..."
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These include: This article cites 38 articles, 25 of which can be accessed free at:
Tech Insight High-throughput methods of regulatory element discovery
"... Recent technological advances have allowed the applica-tion of high-throughput methods to the identification of transcription factor binding regions on a genomic scale. The combination of chromatin immunoprecipitation (ChIP) ..."
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Recent technological advances have allowed the applica-tion of high-throughput methods to the identification of transcription factor binding regions on a genomic scale. The combination of chromatin immunoprecipitation (ChIP)
Generic High-Throughput Methods for Multilingual Sentiment Detection
"... Abstract—Digital ecosystems typically involve a large number of participants from different sectors who generate rapidly growing archives of unstructured text. Measuring the frequency of certain terms to determine the popularity of a topic is comparably straightforward. Detecting sentiment expressed ..."
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and English document repositories. Digital ecosystems may contain millions of frequently updated documents, requiring sentiment detection methods that maximize throughput. The ideal combination of high-throughput techniques and more accurate (but slower) approaches depends on the specific requirements
High Throughput Method for Protein Structure Prediction
"... to make digital or hard copies of portions of this work for personal or classroom use is granted provided that the copies are not made or distributed for profit or commercial advantage and that copies bear this notice and the full citation on the first page. To copy otherwise requires prior specific ..."
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to make digital or hard copies of portions of this work for personal or classroom use is granted provided that the copies are not made or distributed for profit or commercial advantage and that copies bear this notice and the full citation on the first page. To copy otherwise requires prior specific permission by the publisher mentioned above.
J: A high throughput method for genome-wide analysis of retroviral integration
- Nucleic Acids Res
"... Retroviral and lentiviral vectors integrate their DNA into the host cell genome leading to stable transgene expression. Integration preferentially occurs in the proximity of active genes, and may in some case disturb their activity, with adverse toxic consequences. To efficiently analyze high number ..."
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Cited by 1 (0 self)
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numbers of lentiviral insertion sites in the DNA of transduced cells, we developed an improved high-throughput method called vector integration tag analysis (VITA). VITA is based on the identification of Genomic Tags associated to the insertion sites, which are used as signatures of the integration events
Muscle: multiple sequence alignment with high accuracy and high throughput
- NUCLEIC ACIDS RES
, 2004
"... We describe MUSCLE, a new computer program for creating multiple alignments of protein sequences. Elements of the algorithm include fast distance estimation using kmer counting, progressive alignment using a new profile function we call the logexpectation score, and refinement using tree-dependent r ..."
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Cited by 2509 (7 self)
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, MUSCLE achieves average accuracy statistically indistinguishable from T-Coffee and MAFFT, and is the fastest of the tested methods for large numbers of sequences, aligning 5000 sequences of average length 350 in 7 min on a current desktop computer. The MUSCLE program, source code and PREFAB test data
High-Throughput Method for Microfluidic Placement of Cells in Micropatterned Tissues
"... Recent studies have shown that cell shape and tissue structure can dictate functional behavior in engineered tissues 1. One method for controlling tissue structure in vitro is microcontact printing, where extracellular matrix proteins are patterned on a substrate to construct arrays of single cells ..."
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, which creates more replicable high-fidelity tissues. However, the current method is low-throughput and labor intensive. Here, we present a scalable system of multiple microfluidic devices for parallel cell seeding. This high-throughput, precise approach reduces experimental variation, making biochemical
1 High-throughput Method for Determination of Seed Paternity by Microsatellite Markers
, 2013
"... [Abstract] In this protocol, determination of seed paternity by microsatellite markers in Nicotiana attenuata is described. However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a closely related species N. tabacum (Bin ..."
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(Bindler et al., 2007). This is a high-throughput protocol optimized and streamlined for one skilled person to process 384 (96 x 4) seeds in 5 days, from DNA isolation (from seedlings) to paternity assessment by microsatellite genotype data. Materials and Reagents 1. Agencourt Chloropure Kit (this kit
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