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IV. Metabolic Requirements

by Pancreatic Exocrine Cell, James D. Jamieson, George E. Palade
"... Since in the pancreatic exocrine cell synthesis and intracellular transport of secretory proteins can be uncoupled (1), it is possible to examine separately the metabolic requirements of the latter process. To this intent, guinea pig pancreatic slices were pulse labeled with leucine-SH for 3 man and ..."
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Since in the pancreatic exocrine cell synthesis and intracellular transport of secretory proteins can be uncoupled (1), it is possible to examine separately the metabolic requirements of the latter process. To this intent, guinea pig pancreatic slices were pulse labeled with leucine-SH for 3 man

Condensing vacuole conversion and zymogen granule discharge in pancreatic exocrine cells: metabolic studies, d. Cell BtoL

by James D Jamieson, George E Palade , 1971
"... We have examined, in the pancreatic exocrine cell, the metabolic requirements for the conversion of condensing vacuoles into zymogen granules and for the discharge of the contents of zymogen granules. To study condensing vacuole conversion, we pulse labeled guinea pig pancreatic slices for 4 min wit ..."
Abstract - Cited by 12 (3 self) - Add to MetaCart
We have examined, in the pancreatic exocrine cell, the metabolic requirements for the conversion of condensing vacuoles into zymogen granules and for the discharge of the contents of zymogen granules. To study condensing vacuole conversion, we pulse labeled guinea pig pancreatic slices for 4 min

Junctional complexes in various epithelia

by Marilyn G. Farquhar, George E. Palade - J. CELL BIOL , 1963
"... The epithelia of a number of glands and cavitary organs of the rat and guinea pig have been surveyed, and in all cases investigated, a characteristic tripartite junctional complex has been found between adjacent cells. Although the complex differs in precise arrangement from one organ to another, it ..."
Abstract - Cited by 265 (4 self) - Add to MetaCart
The epithelia of a number of glands and cavitary organs of the rat and guinea pig have been surveyed, and in all cases investigated, a characteristic tripartite junctional complex has been found between adjacent cells. Although the complex differs in precise arrangement from one organ to another

Transport from Protein Synthesis

by Pancreatic Exocrine Cell, James D. Jamieson, George E. Palade
"... Experiments have been carried out to determine whether intracellular transport of pancreatic secretory proteins is obligatorily coupled to protein synthesis or whether it is a separable process which can be independently regulated. To this intent, guinea pig pancreatic slices were pulse labeled with ..."
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Experiments have been carried out to determine whether intracellular transport of pancreatic secretory proteins is obligatorily coupled to protein synthesis or whether it is a separable process which can be independently regulated. To this intent, guinea pig pancreatic slices were pulse labeled

RADIOAUTOGRAPHIC LOCALIZATION OF THE INCREASED SYNTHESIS OF PHOSPHATIDYLINOSITOL IN RESPONSE TO PANCREOZYMIN OR ACETYLCHOLINE IN GUINEA PIG PANCREAS SLICES

by Lowell E. Hokin, Dorothy Huebner
"... A technique is described for measuring the incorporation of my0-inositol-2-3H into the lipid of various rcgions of the guinea pig pancreatic acinar ccll by radioautography. Stimulation of crLzymc secretion with either pancrcozymin or acctylcholine was associated with increased graining in both the b ..."
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A technique is described for measuring the incorporation of my0-inositol-2-3H into the lipid of various rcgions of the guinea pig pancreatic acinar ccll by radioautography. Stimulation of crLzymc secretion with either pancrcozymin or acctylcholine was associated with increased graining in both

Intracellular transport of secretory proteins in the pancreatic exocrine cell. II. Transport to condensing vacuoles and zyrnogen granules. or. Cell Biol

by James D. Jamieson, George E. Palade , 1967
"... It has been established by electron microscopic radioautography of guinea pig pancreatic exocrine cells (Caro and Palade, 1964) that secretory proteins are transported from the elements of the rough-surfaced endoplasmic reticulum (ER) to condensing vacuoles of the Golgi complex possibly via small ve ..."
Abstract - Cited by 91 (4 self) - Add to MetaCart
vesicles located in the periphery of the complex. To define more clearly the role of these vesicles in the intracellular transport of secretory proteins, we have investigated the secretory cycle of the guinea pig pancreas by cell fractionation procedures applied to pancreatic slices incubated in vitro

aldehyde oxidase and liver slices: inhibition by isovanillin

by Georgios I. Panoutsopoulos, Christine Beedham
"... Enzymatic oxidation of phthalazine with guinea pig liver ..."
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Enzymatic oxidation of phthalazine with guinea pig liver

Sodium extrusion and potassium uptake in guinea pig kidney cortex slices

by Guillermo Whittembury - S. WILLIS Ion Transport in Hibernator Kidneys , 1965
"... ABSTRACT Slices from the cortex corticis of the guinea pig kidney were immersed in a chilled solution without K and then reimmersed in warmer solutions. The Na and K concentrations and the membrane potential V,,, were then studied as a function of the Na and K concentrations of the reimmersion fluid ..."
Abstract - Cited by 3 (0 self) - Add to MetaCart
ABSTRACT Slices from the cortex corticis of the guinea pig kidney were immersed in a chilled solution without K and then reimmersed in warmer solutions. The Na and K concentrations and the membrane potential V,,, were then studied as a function of the Na and K concentrations of the reimmersion

Protocatechuic Aldehyde with Freshly Prepared Guinea Pig Liver Slices

by Cell Physiol, Biochem Accepted September, Cellular Physiology, Enzymatic Oxidation Of Vanillin, Georgios I. Panoutsopoulos, Christine Beedham, Key Words
"... Background/Aims: The oxidation of xenobiotic-derived aromatic aldehydes with freshly prepared liver slices has not been previously reported. The present investigation compares the relative contribution of aldehyde oxidase, xanthine oxidase and aldehyde dehydrogenase activities in the oxidation of va ..."
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Background/Aims: The oxidation of xenobiotic-derived aromatic aldehydes with freshly prepared liver slices has not been previously reported. The present investigation compares the relative contribution of aldehyde oxidase, xanthine oxidase and aldehyde dehydrogenase activities in the oxidation

Cell fractionation studies on the guinea pig pancreas

by George A. Scheele, George E. Palade, Alan M. Tartakoff - J Cell Biol , 1978
"... A double-label protocol was used to estimate the extent of leakage and relocation artifacts that affect exocrine pancreatic proteins in cell fractionation experiments. Guinea pig pancreatic lobules were pulsed in vitro with a mixture of ~4C-amino acids to enable the lobules to produce and process en ..."
Abstract - Cited by 9 (3 self) - Add to MetaCart
A double-label protocol was used to estimate the extent of leakage and relocation artifacts that affect exocrine pancreatic proteins in cell fractionation experiments. Guinea pig pancreatic lobules were pulsed in vitro with a mixture of ~4C-amino acids to enable the lobules to produce and process
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