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Susceptibility of Vibrio parahaemolyticus to Tris-Dependent DNA Degradation

by unknown authors
"... during Pulsed-Field Gel Electrophoresis This study reports a characteristic of some Vibrio parahae-molyticus strains which are susceptible to electrophoresis-re-lated DNA-degradation (ERDD) by pulsed-field gel electro-phoresis (PFGE) in Tris-borate EDTA (TBE) buffer. V. parahaemolyticus is a halophi ..."
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during Pulsed-Field Gel Electrophoresis This study reports a characteristic of some Vibrio parahae-molyticus strains which are susceptible to electrophoresis-re-lated DNA-degradation (ERDD) by pulsed-field gel electro-phoresis (PFGE) in Tris-borate EDTA (TBE) buffer. V. parahaemolyticus is a

DOI: 10.4161/psb.6.9.16595

by Addendum To Matsushima R, Tang Ly, Zhang L, Yamada H, Twell D, Sakamoto W. A Conserved
"... DNA degradation ..."
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DNA degradation

Apoptotic DNA Degradation into Oligonucleosomal Fragments, but Not Apoptotic Nuclear Morphology, Relies on

by Victoria Iglesias-guimarais, Estel Gil-guiñon, Gisela Gabernet, Mercè García-belinchón, María Sánchez-osuna, Elisenda Casanelles, Joan X. Comella, Victor J. Yuste
"... Background: Apoptotic oligonucleosomal DNA degradation is mediated by DFF40/CAD endonuclease. Results: Poor DFF40/CAD expression in the cytosol coupled to the caspase-dependent cytosolic processing of ICADL/S impair oligonucleosomal DNA degradation in SK-N-AS cells. Conclusion: Oligonucleosomal DNA ..."
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Background: Apoptotic oligonucleosomal DNA degradation is mediated by DFF40/CAD endonuclease. Results: Poor DFF40/CAD expression in the cytosol coupled to the caspase-dependent cytosolic processing of ICADL/S impair oligonucleosomal DNA degradation in SK-N-AS cells. Conclusion: Oligonucleosomal DNA

Post Mortem DNA Degradation of Human Tissue Experimentally Mummified in Salt

by Natallia Shved, Cordula Haas, Guelfirde Akguel, Katja Paulsen, Abigail Bouwman , 2014
"... Mummified human tissues are of great interest in forensics and biomolecular archaeology. The aim of this study was to analyse post mortem DNA alterations in soft tissues in order to improve our knowledge of the patterns of DNA degradation that occur during salt mummification. In this study, the lowe ..."
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Mummified human tissues are of great interest in forensics and biomolecular archaeology. The aim of this study was to analyse post mortem DNA alterations in soft tissues in order to improve our knowledge of the patterns of DNA degradation that occur during salt mummification. In this study

DNase 2 Is the Main DNA-Degrading Enzyme of the Stratum Corneum

by Heinz Fischer, Jennifer Scherz, Ra Szabo, Michael Mildner, Charaf Benarafa, Alicia Torriglia, Erwin Tschachler, Leopold Eckhart , 2010
"... The cornified layer, the stratum corneum, of the epidermis is an efficient barrier to the passage of genetic material, i.e. nucleic acids. It contains enzymes that degrade RNA and DNA which originate from either the living part of the epidermis or from infectious agents of the environment. However, ..."
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The cornified layer, the stratum corneum, of the epidermis is an efficient barrier to the passage of genetic material, i.e. nucleic acids. It contains enzymes that degrade RNA and DNA which originate from either the living part of the epidermis or from infectious agents of the environment. However

Effects of preservation and storage of microcrustaceans in RNAlater on RNA and DNA degradation

by Elena Gorokhova
"... Methods of preserving nucleic acids are increasingly in demand because of the recent advances in molecular and biochemical approaches to ecology. The RNA storage reagent RNAlater ® was tested as an alternative method to deep-freezing for preserving RNA and DNA in zooplankton. Artemia spp. nauplii we ..."
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no significant changes in DNA were detected. In samples stored in RNAlater without refrigeration, RNA did not exhibit significant decrease for at least 1 month, and DNA for at least 2 months. As RNA and DNA degraded at roughly the same rate, this resulted in little or no changes in RNA:DNA ratios

DNA FROM HERBARIUM SPECIMENS: II. CORRELATION OF DNA DEGRADATION WITH HUMIDITY

by Robert P Adams
"... ABSTRACT Comparisons were made of DNA extracted from leaves of Juniperus virginiana stored in 100, 75, 70, 54 and 23% humidity at 21ºC for up to 12 months. Fungal growth was observed in the 100, 75 and 70% humidity tests that resulted in the degradation of the juniper DNA. The DNA in leaves stored ..."
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ABSTRACT Comparisons were made of DNA extracted from leaves of Juniperus virginiana stored in 100, 75, 70, 54 and 23% humidity at 21ºC for up to 12 months. Fungal growth was observed in the 100, 75 and 70% humidity tests that resulted in the degradation of the juniper DNA. The DNA in leaves stored

The Roles and Acting Mechanism of Caenorhabditis elegans DNase II Genes in Apoptotic DNA Degradation and Development

by Huey-jen Lai, Szecheng J. Lo, Eriko Kage-nakadai, Shohei Mitani, Ding Xue
"... DNase II enzymes are acidic endonucleases that have been implicated in mediating apoptotic DNA degradation, a critical cell death execution event. C. elegans genome contains three DNase II homologues, NUC-1, CRN-6, and CRN-7, but their expression patterns, acting sites, and roles in apoptotic DNA de ..."
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DNase II enzymes are acidic endonucleases that have been implicated in mediating apoptotic DNA degradation, a critical cell death execution event. C. elegans genome contains three DNase II homologues, NUC-1, CRN-6, and CRN-7, but their expression patterns, acting sites, and roles in apoptotic DNA

NUC-1, a Caenorhabditis elegans DNase II homolog, functions in an intermediate step of DNA degradation during apoptosis

by Yi-chun Wu, Gillian M. Stanfield, H. Robert Horvitz - Genes Dev , 2000
"... One hallmark of apoptosis is the degradation of chromosomal DNA. We cloned the Caenorhabditis elegans gene nuc-1, which is involved in the degradation of the DNA of apoptotic cells, and found that nuc-1 encodes a homolog of mammalian DNase II. We used the TUNEL technique to assay DNA degradation in ..."
Abstract - Cited by 14 (1 self) - Add to MetaCart
One hallmark of apoptosis is the degradation of chromosomal DNA. We cloned the Caenorhabditis elegans gene nuc-1, which is involved in the degradation of the DNA of apoptotic cells, and found that nuc-1 encodes a homolog of mammalian DNase II. We used the TUNEL technique to assay DNA degradation

Functional genomic analysis of apoptotic DNA degradation in C. elegans

by Jay Zachary Parrish, Ding Xue - Mol Cell , 2003
"... have been implicated in mediating apoptotic DNA deg-University of Colorado ..."
Abstract - Cited by 12 (3 self) - Add to MetaCart
have been implicated in mediating apoptotic DNA deg-University of Colorado
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