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Journal of Neuroscience Methods 157 (2006) 195–207 Expression profiling of intermingled long-range projection neurons harvested by laser capture microdissection

by Anthony J. Lombardino, Moritz Hertel, Xiao-ching Li, Bhagwattie Haripal, Laurel Martin-harris, Eben Pariser, O Nottebohm
"... Gene expression data are most useful if they can be associated with specific cell types. This is particularly so in an organ such as the brain, where many different cell types lie in close proximity to each other. We used zebra finches (Taeniopygia guttata), fluorescent tracers and laser capture mic ..."
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RNA was obtained, too, from backfilled neurons birth-dated with bromodeoxyuridine (BrdU). We also quantified changes in the levels of three genes after singing behavior using qPCR. Thus, we have brought together a combination of techniques allowing for the molecular profiling of intermingled

synthase and

by Laura Strittmatter, Yang Li, Nathan J. Nakatsuka, Sarah E. Calvo, Zenon Grabarek, Vamsi K. Mootha , 2013
"... is a polymorphic human enzyme with malate ..."
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is a polymorphic human enzyme with malate

unknown title

by Laura Strittmatter, Yang Li, Nathan Nakatsuka, Sarah Calvo
"... CLYBL is a polymorphic human enzyme with malate synthase and -methylmalate synthase activity ..."
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CLYBL is a polymorphic human enzyme with malate synthase and -methylmalate synthase activity

AGR 05

by Università Degli, Studi Della Tuscia, Dottorato Di Ricerca, Candidato Tutori, Alessandra Lagomarsino, Prof Paolo, De Angelis, Prof Stefano Grego, Prof Paolo, De Angelis
"... Responses of soil biological processes to elevated atmospheric [CO2] and nitrogen addition in a poplar plantation ..."
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Responses of soil biological processes to elevated atmospheric [CO2] and nitrogen addition in a poplar plantation

Copper transporter 2 regulates the cellular accumulation and cytotoxicity of Cisplatin and

by Brian G Blair , Christophera Larson , Roohangiz Safaei , Stephen B Howell , Cancertherapy : Preclinical , Stephen B Howell , 2009
"... Abstract Purpose: Copper transporter 2 (CTR2) is known to mediate the uptake of Cu +1 by mammalian cells. Several other Cu transporters, including the influx transporter CTR1 and the two efflux transporters ATP7A and ATP7B, also regulate sensitivity to the platinum-containing drugs. We sought to de ..."
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RNA levels were measured using a quantitative PCR (qPCR) method of detection of relative amounts of first-strand cDNA. cDNA was generated from mRNA isolated using Trizol (Invitrogen). Purified mRNA was converted to cDNA using oligo(dT) 20 priming and the SuperScript III First-Strand kit (Invitrogen). qPCR

unknown title

by Ida O
"... 5 nited ..."
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Abstract not found

Triamcinolone Acetonide Decreases Outflow Facility in C57BL/6 Mouse Eyes

by Sandeep Kumar , Shaily Shah , Emily Rose Deutsch , Hai Michael Tang , John Danias , 2013
"... PURPOSE. To determine the effect of triamcinolone acetonide (TA) on outflow facility in mice. METHODS. Animals received 20 lL of TA (40 mg/mL) suspension subconjunctivally either bilaterally or unilaterally and were euthanized after either 1 week or 3 weeks. Before mice were killed, IOP was measure ..."
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was investigated in a subset of eyes using quantitative PCR (qPCR). RESULTS. Outflow facility of eyes in animals receiving bilateral TA injection (TA BL ) and TA-treated eyes of animals receiving unilateral injection (TA UL ) was significantly decreased compared to naïve control eyes (C naive ) after 1 week and 3

ZEB1 enhances transendothelial migration and represses the epithelial phenotype of prostate cancer cells,”

by Justin M Drake , Garth Strohbehn , Thomas B Bair , Jessica G Moreland , Michael D Henry , Facility † Dna , Roy J , Lucille A Carver - Molecular Biology of the Cell, , 2009
"... Metastatic colonization involves cancer cell lodgment or adherence in the microvasculature and subsequent migration of those cells across the endothelium into a secondary organ site. To study this process further, we analyzed transendothelial migration of human PC-3 prostate cancer cells in vitro. ..."
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difference was calculated by two-way ANOVA analysis with false discovery rate (FDR). An FDR of q Ͻ 0.05 was used as a significance threshold; 752 probe sets (1.38%) showed Ϯ2-fold change between PC-3 and the TEM4-18/TEM2-5 cell lines. For gene ontology analysis comparing PC-3 and TEM4-18, an FDR of q Ͻ 0

Fibrocartilage tissue engineering: the role of the stress environment on cell morphology and matrix expression." Tissue engineering. Part A

by Ph.D Stavros Thomopoulos , M.S Rosalina Das , Ph.D Victor Birman , Ph.D Lester Smith , Katherine Ku , B S , Ph.D Elliott L Elson , Ph.D Kenneth M Pryse , Ph.D Juan Pablo Marquez , Ph.D Guy M Genin , 2011
"... Although much is known about the effects of uniaxial mechanical loading on fibrocartilage development, the stress fields to which fibrocartilaginous regions are subjected to during development are mutiaxial. That fibrocartilage develops at tendon-to-bone attachments and in compressive regions of te ..."
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(Thermo Scientific). Five hundred nanograms of RNA was reverse transcribed to cDNA using the Superscript III RT kit (Invitrogen) following manufacturer's instructions. qPCR reactions were performed using Sybr Green chemistry on a 7300 sequence detection system (Applied Biosystems). Forward
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