Atwbc18 (SALK_100187) and Atwbc16 (SALK_119868) that share over 76 % similarity in amino acid sequence with Atwbc19 (see Supplementary Fig. 5). The mutants were T-DNA insertional mutants transformed with plasmid pROK22 containing an nptII gene, under the control of the nopaline synthase (nos) promoter

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loloo 1jU=125 ilE±I MICROCOPY RESOLUTION TEST CHART

United Kingdom Approved for Public Release; distribution unlimited. BUG fOlMS?T IMEPBGIED 4 20000816 038 ^ü^u,rrr-i,nM^M^^i^i^ ßMlifeififti iBIic rep< ^- _ REPORTDOCUMENTATION PAGE ^ApP">Vcd Ehereport ^ burden for (his colle^T^brrnation is estimated to average 1 hourperVes^e includi^TT—f-I QMB N°- 0704-0188 *f ^ fr»r infnn«.) I — _ ^ _. •. _ r l Uli. 3iSiSiiäpi^^^=^==£iH %LE AND SUBTITLE _____

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project reviewed existing laboratory methods for accurately describing the constitutive behavior of the mixes used in the Commonwealth of Virginia. Indirect tensile (IDT) strength, resilient modulus, static creep in the IDT and uniaxial modes, flexural beam fatigue, and dynamic modulus tests were conducted on two typical mixes used in Virginia: SM-9.5A (surface mix) and BM-25.0 (base mix). The tests conducted produced a wealth of data on typical values for the properties of the two mixes studied over a wide range of temperatures and loading frequencies. The results suggest that the IDT strength test is an effective test to characterize the tensile strength of hot-mix asphalt (HMA), especially for thermal cracking evaluation. The resilient modulus test and the static creep test in the IDT setup are practical and simple to perform, but the analysis of the measurements is complicated, and the variability of the results is high. The compressive uniaxial dynamic modulus and the uniaxial static creep tests were found to be simple to conduct and to analyze because of the homogeneous state of stress in the specimen during testing. The flexural fatigue test was time consuming, but the test produces valuable information about the fatigue properties of hot-mix asphalt. The investigation also found good correlations among the IDT strength, resilient modulus, and dynamic modulus results. A variety of tests is recommended for characterizing the mechanistic-empirical pavement analysis and design. These tests